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random primer sequence  (TaKaRa)


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    Structured Review

    TaKaRa random primer sequence
    Random Primer Sequence, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+sequence/Random+Primer/us12428686-274-1-62
    Average 96 stars, based on 6510 article reviews
    random primer sequence - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: Identification of quinolone derivatives as effective anti-Dengue virus agents.
    Article Snippet: Dengue virus (DENV) infection is one of the most important infectious diseases in tropical and subtropical regions around the world.. Previously, we performed an initial phenotypic screening of 7000 compounds using DENV type 2 (DENV2)-infected BHK-21 cells to identify small molecules which could inhibit virus replication.. In this study, we describe two novel compounds with anti-DENV2 activity, tentatively named Compound-X and Compound-Y.

    Article Title: Method for fragmenting double-stranded RNA and use of the same
    Article Snippet: The obtained RNA fraction was treated with DNase I (Takara, Otsu, Japan) to remove remaining DNA. .. DsDNA having a sequence corresponding to RNA was synthesized with random primers (9-mer) by using PrimeScript Double Strand cDNA Synthesis Kit (Takara). ..

    Article Title: Molecular cloning, expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica
    Article Snippet: The Guanine nucleotide exchange factor Vav2 (Vav2) is a member of the Vav family that serves as an important regulators for the Rho family of Ras-related GTPases.. In the current study, an ortholog (Lj-Vav2) of Vav2 was identified in the lamprey (Lampetra japonica).. To elucidate the phylogenetic relationship of Vav2, the metazoan genome databases were analyzed to mine the ortholog of Vav.

    Sequencing:

    Article Title: Identification of quinolone derivatives as effective anti-Dengue virus agents.
    Article Snippet: Dengue virus (DENV) infection is one of the most important infectious diseases in tropical and subtropical regions around the world.. Previously, we performed an initial phenotypic screening of 7000 compounds using DENV type 2 (DENV2)-infected BHK-21 cells to identify small molecules which could inhibit virus replication.. In this study, we describe two novel compounds with anti-DENV2 activity, tentatively named Compound-X and Compound-Y.

    Article Title: Method for fragmenting double-stranded RNA and use of the same
    Article Snippet: The obtained RNA fraction was treated with DNase I (Takara, Otsu, Japan) to remove remaining DNA. .. DsDNA having a sequence corresponding to RNA was synthesized with random primers (9-mer) by using PrimeScript Double Strand cDNA Synthesis Kit (Takara). ..

    Article Title: Molecular cloning, expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica
    Article Snippet: The Guanine nucleotide exchange factor Vav2 (Vav2) is a member of the Vav family that serves as an important regulators for the Rho family of Ras-related GTPases.. In the current study, an ortholog (Lj-Vav2) of Vav2 was identified in the lamprey (Lampetra japonica).. To elucidate the phylogenetic relationship of Vav2, the metazoan genome databases were analyzed to mine the ortholog of Vav.

    Article Title: Set of random primers and method for preparing DNA library using the same
    Article Snippet: .. 3.8 Random Primer Sequence To the genomic DNA described in 2. above (30 ng, NiF8-derived genomic DNA), a set of primers selected from the 5 sets of random primers shown in Tables 4 to 8 was added to result in the final concentration of 60 microM therein, a 0.2 mM dNTP mixture, 1.0 mM MgCl2, and 1.25 units of DNA polymerase (PrimeSTAR, TAKARA) were added thereto, and a reaction solution was prepared while adjusting the final reaction level to 50 microliters. ..

    Article Title: Molecular characterization of the porcine S100A6 gene and analysis of its expression in pigs infected with highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV).
    Article Snippet: Our previous microarray study revealed that S100A6 was significantly upregulated in porcine alveolar macrophages (PAMs) infected with highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV).. In the present study, we cloned both cDNA and genomic DNA sequences of the gene.. Transient transfection indicated that the porcine S100A6 protein was located in the nucleus and cytoplasm.

    Random Hexamer:

    Article Title: Identification of quinolone derivatives as effective anti-Dengue virus agents.
    Article Snippet: Dengue virus (DENV) infection is one of the most important infectious diseases in tropical and subtropical regions around the world.. Previously, we performed an initial phenotypic screening of 7000 compounds using DENV type 2 (DENV2)-infected BHK-21 cells to identify small molecules which could inhibit virus replication.. In this study, we describe two novel compounds with anti-DENV2 activity, tentatively named Compound-X and Compound-Y.

    cDNA Synthesis:

    Article Title: Identification of quinolone derivatives as effective anti-Dengue virus agents.
    Article Snippet: Dengue virus (DENV) infection is one of the most important infectious diseases in tropical and subtropical regions around the world.. Previously, we performed an initial phenotypic screening of 7000 compounds using DENV type 2 (DENV2)-infected BHK-21 cells to identify small molecules which could inhibit virus replication.. In this study, we describe two novel compounds with anti-DENV2 activity, tentatively named Compound-X and Compound-Y.

    Article Title: Method for fragmenting double-stranded RNA and use of the same
    Article Snippet: The obtained RNA fraction was treated with DNase I (Takara, Otsu, Japan) to remove remaining DNA. .. DsDNA having a sequence corresponding to RNA was synthesized with random primers (9-mer) by using PrimeScript Double Strand cDNA Synthesis Kit (Takara). ..

    Reverse Transcription:

    Article Title: Molecular cloning, expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey, Lampetra japonica
    Article Snippet: The Guanine nucleotide exchange factor Vav2 (Vav2) is a member of the Vav family that serves as an important regulators for the Rho family of Ras-related GTPases.. In the current study, an ortholog (Lj-Vav2) of Vav2 was identified in the lamprey (Lampetra japonica).. To elucidate the phylogenetic relationship of Vav2, the metazoan genome databases were analyzed to mine the ortholog of Vav.

    Article Title: Inhibition of mucin secretion via glucocorticoid-induced regulation of calcium-related proteins in mouse lung.
    Article Snippet: Mouse lung was washed with cold sterile saline and homogenized in TRIzol (Ambion, Austin, TX) with a bullet blender, and total RNA concentration of the homogenate was determined by absorbance at 260 nm by using an Epoch Microplate Spectrophotometer (BioTek Instruments, Winooski, VT). .. Subsequently, 1 g of RNA was transcribed by using Moloney murine leukemia virus reverse transcriptase (iNtRON Bio, Gyeonggi-do, Republic of Korea) with a random primer (9-mer; TaKaRa Bio, Kusatsu, Japan) to produce first-strand complementary DNA (cDNA). .. The cDNA template (2 l) was assayed by using SYBR Premix Ex Taq (TaKaRa Bio) and performing qPCR according to the manufacturer’s protocol with 10 pmol of primers specific for Trpv4, Trpv6, Cabp-9k, Ncx1, Pmca1, Gr , CC10, SP-C, Serca2a, and Ryr2.

    Concentration Assay:

    Article Title: Set of random primers and method for preparing DNA library using the same
    Article Snippet: .. 3.8 Random Primer Sequence To the genomic DNA described in 2. above (30 ng, NiF8-derived genomic DNA), a set of primers selected from the 5 sets of random primers shown in Tables 4 to 8 was added to result in the final concentration of 60 microM therein, a 0.2 mM dNTP mixture, 1.0 mM MgCl2, and 1.25 units of DNA polymerase (PrimeSTAR, TAKARA) were added thereto, and a reaction solution was prepared while adjusting the final reaction level to 50 microliters. ..

    Virus:

    Article Title: Inhibition of mucin secretion via glucocorticoid-induced regulation of calcium-related proteins in mouse lung.
    Article Snippet: Mouse lung was washed with cold sterile saline and homogenized in TRIzol (Ambion, Austin, TX) with a bullet blender, and total RNA concentration of the homogenate was determined by absorbance at 260 nm by using an Epoch Microplate Spectrophotometer (BioTek Instruments, Winooski, VT). .. Subsequently, 1 g of RNA was transcribed by using Moloney murine leukemia virus reverse transcriptase (iNtRON Bio, Gyeonggi-do, Republic of Korea) with a random primer (9-mer; TaKaRa Bio, Kusatsu, Japan) to produce first-strand complementary DNA (cDNA). .. The cDNA template (2 l) was assayed by using SYBR Premix Ex Taq (TaKaRa Bio) and performing qPCR according to the manufacturer’s protocol with 10 pmol of primers specific for Trpv4, Trpv6, Cabp-9k, Ncx1, Pmca1, Gr , CC10, SP-C, Serca2a, and Ryr2.

    Cloning:

    Article Title: Bacillus subtilis engineered for aerospace medicine: a platform for off-planet production of pharmaceutical peptides
    Article Snippet: .. A library of 173 unique signal peptides found in Bacillus subtilis strain 168 was inserted into this linearized backbone using In-Fusion cloning and the Signal Peptide Library DNA purchased from TAKARA Bio as a part of their B. subtilis Secretory Protein Expression System, according to the manufacturer’s protocols. ..

    Nested PCR:

    Article Title: Molecular characterization of the porcine S100A6 gene and analysis of its expression in pigs infected with highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV).
    Article Snippet: Our previous microarray study revealed that S100A6 was significantly upregulated in porcine alveolar macrophages (PAMs) infected with highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV).. In the present study, we cloned both cDNA and genomic DNA sequences of the gene.. Transient transfection indicated that the porcine S100A6 protein was located in the nucleus and cytoplasm.



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